Cordyceps Recipe & Tek

-(Instructional Video Coming Soon)-

*Be sure to read everything before starting your project.*

Test your master Liquid Culture (LC) on agar to ensure cleanliness.
2. Create more LC by using this recipe below and pressure cooking your 500ml filled jar for 25 minutes at 15psi.

LC Recipe:

500ml water

1tsp dextrose

1/4tsp LME

3. After the LC jars are cooled, inculcate them with your master LC. Shake the jar or spin your magnetic stir bar periodically.

4. After your jars are full of mycelium (~3 days) withdraw a sample and test on agar.

5. Create your rice-broth substrate using the recipe below. You want 1 inch of substrate or under. Sterilizable Polypropylene 5 (PP5) containers from Dollar Tree work perfectly for this tek. (See photo below).

Broth Recipe:

1 liter water

16g LME (light malt extract)

10g corn starch

4.5 gypsum

3.5 yeast

Use 1 cup of rice to 500ml of the broth (above recipe). Then PC for 1hr.

6. Once the cleanliness of your expanded culture is confirmed, you can inoculate 60ml of your culture directly to your sterilized and cooled rice-broth substrate. (There will be pooling, this is good!). Be sure to spread the LC all over your substrate by squirting it everywhere or mixing it into your substrate with a sterile utensil such as a spoon. (You can wrap a metal spoon in foil and pre-sterilize it beside your liquid culture back in step number 2). If you decide to mix it with a spoon be sure to mix in front of a laminar flow hood. Pat down and level out your substrate in the container with the spoon.

**Incubate your kit under 70f at all times. LCs should be stored in a fridge.**